Pair Name	Shikonin, 4-hydroxytamoxifen
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	ROS1	hsa6098
	Down-regulation	Expression	PIK3CA	hsa5290
	Down-regulation	Expression	AKT1	hsa207
	Up-regulation	Cleavage	CASP9	hsa842
	Up-regulation	Cleavage	PARP1	hsa142
	Up-regulation	Expression	DIABLO	hsa56616
	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Expression	BAX	hsa581
	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Expression	FAS	hsa355
	Up-regulation	Cleavage	CASP8	hsa841
	Down-regulation	Expression	BID	hsa637
In Vitro Model	MCF-7	Invasive breast carcinoma of no special type	Homo sapiens (Human)	CVCL_0031
	MDA-MB-435S	Amelanotic melanoma	Homo sapiens (Human)	CVCL_0622
In Vivo Model	Portions of the suspension (6×10⁶ cells in 0.1 mL) were subcutaneously injected into the right flanks of each mouse.
Result	The combination of SK and 4-OHT shows highly efficient anticancer effects on breast cancer therapy. SK may be a promising candidate as an adjuvant to 4-OHT for breast cancer treatments, especially for ER- breast cancer.